An Investigation on the Techniques used in Force Calibration using Deadweights and Pressure Piston Gauge

537-543This paper presents two mechanisms for load cell calibrations. The first technique was to use deadweight to generate the required reference load (Applied load). The second method was to use a pressure piston gauge to generate the required calibration force. In both mechanisms, the setup and procedures are described. For verification the results of these methods were compared with international metrology institute calibration results. It was found that deadweight method is the most accurate and the piston gauge is the most realistic at high force values

An Investigation on the Techniques used in Force Calibration using Deadweights and Pressure Piston Gauge

This paper presents two mechanisms for load cell calibrations. The first technique was to use deadweight to generate the required reference load (Applied load). The second method was to use a pressure piston gauge to generate the required calibration force. In both mechanisms, the setup and procedures are described. For verification the results of these methods were compared with international metrology institute calibration results. It was found that deadweight method is the most accurate and the piston gauge is the most realistic at high force values

Application of direct bioautography and SPME-GC-MS for the study of antibacterial chamomile ingredients

The isolation and characterization of antibacterial chamomile components were performed by the use of direct bioautography and solid phase microextraction (SPME)-GC-MS. Four ingredients, active against Vibrio fischeri, were identified as the polyacetylene geometric isomers cis- and trans-spiroethers, the coumarin related herniarin, and the sesquiterpene alcohol (-)-alpha-bisabolol

Studies on a gram-positive hydrogen bacterium, <em>Nocardia opaca</em> strain 1b - I. Description and physiological characterization.

A new hydrogen bacterium has been isolated by enrichment culture on propane. It is a strictly aerobic, Gram-positive, non acid-fast bacterium, characterized by filamentous growth, and has been tentatively assigned to Nocardia opaca (strain 1 b). It grows heterotrophically, on many organic compounds (71 out of 138 tested substrates including organic acids and sugars), on hydrocarbons (C11-C18) as well as under autotrophic conditions (under an atmosphere of hydrogen, oxygen, and carbon dioxide=8:1:1) In the absence of a nitrogen source storage materials, mainly carbohydrates, are accumulated. Hydrogenase is an inducible enzyme. Under appropriate growth conditions the specific hydrogenase activity reaches high values: 2700 enzyme units/g cell protein. The formation of hydrogenase is repressed by fructose. With increasing oxygen concentrations during growth the specific hydrogenase activity decreases. In resting cell oxygen progressively inhibits the oxyhydrogen reaction. Cell-free extracts of autotrophically grown cells are able to reduce oxygen benzyl-and methyl viologen, dichlorphenolindophenol, methylene blue and nicotinamide adeninedinucleotide with hydrogen

Spatial Analysis of Soil Properties and Site-Specific Management Zone Delineation for the South Hail Region, Saudi Arabia

Sustainable soil management with the appropriate understanding of soil characteristics is vital in maintaining and improving agriculture soil management. The objectives of the present study are to characterize the spatial variability of soil using the GIS technique and used agglomerative hierarchical clustering (AHC) for the delineation of management zones (MZs) for precision agriculture. A total of 111 soil samples were collected from 37 soil profiles in systematic depths (0–50, 50–100, and 100–150 cm) from the South Hail region, KSA. Samples were analyzed for pH, ECe, CaCO3, available macro and micronutrients, and hydrological properties. The best fit models, using ArcGIS software, were J-Bessel for pH, Clay, bulk density (BD), and available water (AW); K-Bessel for EC and available N; Stable for CaCO3, P, K, Fe, Zn, Sand, field capacity (FC) and saturated hydraulic conductivity (Ks); Spherical for Mn and Cu; Gaussian for saturation percentage (SP); whereas exponential for permanent wilting point (PWP). The principal component analysis (PCA) resulted in six principal components (PCs) explaining 79.75% of the total variance of soil properties. The PC1 was strongly influenced by soil BD, FC, clay, PWP, Ks, and sand. PC2 was dominated by N, ECe, and CaCO3; PC3 was dominated by pH; PC4 was dominated primarily by K and P, PC5 was mainly dominated by Fe; Mn, and Cu, and PC6 was mainly dominated by SP and Zn. Based on AHC, four soil management zones (MZs) cover 77.94, 14.10, 7.11 and 0.85% of the studied area. Management zone 1 (MZ1) and Management zone 3 (MZ3) are classified as moderately saline while Management zone 2 (MZ2) is classified as highly saline soils, greater than the limiting critical value for the sensitive crops. The potential solutions to reduce salinization in the area include: reducing irrigation, moving to salt-tolerant crops or applying humic acids to fix anions and cations and eliminate them from the root zone of the plants. Treating the area with diluted sulfuric acid to remove salts and reduce ECe to less than 2 dSm−1, to get maximum productivity. This finding is diagnostic for determining the amount of fertilizer and irrigation water to be applied to soils in different management zones. Its emphasis’s the importance of site-specific management for long-term crop productivity and, as a result, reducing environmental hazards caused by uneven fertilizers and water applications

Studies on a gram-positive hydrogen bacterium, <em>Nocardia opaca</em> 1 b : III. Purification, stability and some properties of the soluble hydrogen dehydrogenase.

Nocardia opaca strain 1 b has a NAD-dependent hydrogenase (hydrogen dehydrogenase). The enzyme has been purified from autotrophically grown cells and tested for optimal assay conditions and stability. The purification procedure involved protamine sulfate treatment, ammonium sulfate precipitation, and separation by DEAE-cellulose and Sephadex G-200 chromatography and resulted in a 63-fold increase of specific activity at a 11.7% enzyme recovery. The final specific activity was 103 &mu;moles H2/min&middot;mg protein. The purified enzyme was dependent on nickel and magnesium ions at 0.5 and 5.0 mM concentrations, respectively, as well as flavin mononucleotide at a 5-10 &mu;M concentration. Straight enzyme kinetics were achieved by preincubating the enzyme in the presence of NADH2. A high stability of the enzyme was observed in 0.1 M potassium phosphate buffer, pH 6.5, in the presence of 0.5 mM nickel and 5 mM magnesium ions under hydrogen atmosphere. Even under air the enzyme was remarkably stable, although less than under hydrogen. From double reciprocal plots of substrate saturation curves the Michaelis-Menten constants were calculated: For saturating NAD-concentration the Kmwas 0.063 mM H2 and for saturating hydrogen concentration the Kmwas 0.123 mM NAD

Spatial Analysis of Soil Properties and Site-Specific Management Zone Delineation for the South Hail Region, Saudi Arabia

Sustainable soil management with the appropriate understanding of soil characteristics is vital in maintaining and improving agriculture soil management. The objectives of the present study are to characterize the spatial variability of soil using the GIS technique and used agglomerative hierarchical clustering (AHC) for the delineation of management zones (MZs) for precision agriculture. A total of 111 soil samples were collected from 37 soil profiles in systematic depths (0&ndash;50, 50&ndash;100, and 100&ndash;150 cm) from the South Hail region, KSA. Samples were analyzed for pH, ECe, CaCO3, available macro and micronutrients, and hydrological properties. The best fit models, using ArcGIS software, were J-Bessel for pH, Clay, bulk density (BD), and available water (AW); K-Bessel for EC and available N; Stable for CaCO3, P, K, Fe, Zn, Sand, field capacity (FC) and saturated hydraulic conductivity (Ks); Spherical for Mn and Cu; Gaussian for saturation percentage (SP); whereas exponential for permanent wilting point (PWP). The principal component analysis (PCA) resulted in six principal components (PCs) explaining 79.75% of the total variance of soil properties. The PC1 was strongly influenced by soil BD, FC, clay, PWP, Ks, and sand. PC2 was dominated by N, ECe, and CaCO3; PC3 was dominated by pH; PC4 was dominated primarily by K and P, PC5 was mainly dominated by Fe; Mn, and Cu, and PC6 was mainly dominated by SP and Zn. Based on AHC, four soil management zones (MZs) cover 77.94, 14.10, 7.11 and 0.85% of the studied area. Management zone 1 (MZ1) and Management zone 3 (MZ3) are classified as moderately saline while Management zone 2 (MZ2) is classified as highly saline soils, greater than the limiting critical value for the sensitive crops. The potential solutions to reduce salinization in the area include: reducing irrigation, moving to salt-tolerant crops or applying humic acids to fix anions and cations and eliminate them from the root zone of the plants. Treating the area with diluted sulfuric acid to remove salts and reduce ECe to less than 2 dSm&minus;1, to get maximum productivity. This finding is diagnostic for determining the amount of fertilizer and irrigation water to be applied to soils in different management zones. Its emphasis&rsquo;s the importance of site-specific management for long-term crop productivity and, as a result, reducing environmental hazards caused by uneven fertilizers and water applications